• 产品名称11，12 DHET ELISA试剂盒
• 检测方法Colorimetric
• 样品类型Urine, Serum, Plasma, Cell culture extracts, Tissue
• 检测类型Competitive
• 灵敏度10 pg/ml
• 范围0.01 ng/ml - 1000 ng/ml
• 实验步骤Multiple steps standard assay
• 产品概述

  Abcam’s11,12 DHETin vitrocompetitive ELISAkit is designed for accurate quantitative measurement in serum, plasma, urine, cell culture andtissue samples following proper isolation and purification.

  This competitive ELISA kit is based on competition between the 11,12 DHET epitope and the 11,12 DHET-HRP conjugate for a limited number of binding sites available from the anti-11,12 DHET antibody; which is coated to the wells of the 96 well ELISA plate. The conjugate concentration is held as a constant in each well, while the concentration of the 11,12 DHET is variable, based on the concentration of the sample or standard. Thus the amount of the 11,12 DHET conjugate which is able to bind to each of the wells is inversely proportional to the concentration of 11,12 DHETin the standard or sample. The amount of the conjugate which is bound to each well is then determined by the amount of color obtained, when TMB is added. The TMB reacts with the HRP available in the well. With the addition of sulfuric acid, the blue colored product is converted into a yellow colored product, which can be read on a plate reader at 450 nm.

  The level of 11,12-DHET or 11,12-DHET epitope has been shown to exhibit correlation with hypertension in rodents (1,2,3). 11,12-DHET is a representative metabolite of cytosolic epoxide hydrolase-mediated metabolism of EETs, which are generated by arachidonic acid epoxygenase activity of cytochrome P450’s (4).

• 经测试应用Competitive ELISAmore details
• 平台Microplate

性能

• 存放说明Store at -20°C. Please refer to protocols.
• 组件1 x 96 tests

  11,12-DHET Standard	1 x 2µl
  11,12-DHET-HRP Conjugates	1 x 12µl
  HRP Buffer	1 x 15ml
  Pre-coated 96-Well Microplate	1 unit
  Sample Dilution Buffer	1 x 25ml
  TMB Substrate	1 x 24ml
  Wash Buffer Solution	1 x 25ml

• 研究领域
  • Signal Transduction
  • Signaling Pathway
  • Lipid Signaling
  • Other
  • Signal Transduction
  • Metabolism
  • Lipid metabolism
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Fatty acids

• 别名
  • 11,12-dihydroxy-5Z,8Z,14Z-eicosatrienoic acid

应用

OurAbpromise guaranteecovers the use ofab175814in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

11,12 DHET ELISA Kit 图像

• Typical Standard Curve

  Representative standard curve using ab175814 11,12 DHET ELISA Kit.