1. INJ Inject-A-Lume h-Coelenterazine 注射型h-腔肠素

【产品货号】Cat#301、CAS#50909-86-9

【产品规格】1kit、10kits

【产品简介】注射型h-腔肠素，纯度高（99%），针对活体研究进行优化。苯甲基腔肠素（h-CTZ)封装于小体积的无菌注射瓶中。每个试剂盒提供两瓶500 µg的注射型h-CTZ和一瓶无菌注射型稀释液。仅供动物使用。

【使用案例】

Rd R W, Oner S S, Lanier S M, et al. Direct Coupling of a Seven-Transmembrane-Span Receptor to a Gαi G-Protein Regulatory Motif Complex.[J]. Molecular Pharmacology, 2015, 88.

Ciruela F, Fernández-Dueñas V. GPCR oligomerization analysis by means of BRET and dFRAP.[J]. Methods in Molecular Biology, 2015, 1272(1272):133-41.

Schena A, Griss R, Johnsson K. Modulating protein activity using tethered ligands with mutually exclusive binding sites.[J]. Nature Communications, 2015, 6.

2. Coelenterazine-SOL in vivo 水溶性腔肠素（活体研究）

【产品货号】cat#3031

【产品规格】500µg、1mg、10mg

【产品简介】天然腔肠素被制定为水溶性的（天然腔肠素+无毒水溶性添加剂），可安全应用于海肾萤光素酶的活体成像。腔肠素经常溶于丙二醇（PG）结合其他醇类的混合溶剂中，应用于活体成像研究。短时间内静脉注射大剂量PG是有毒性的，包括高渗性、增加阴离子代谢性酸中毒（由于乳酸性酸中毒）、急性肾损伤以及血性症候群。使用水溶性腔肠素可避免上述问题，它被制定为等渗压以及易于被肾脏分泌的，从而避免重复性的活体注射。水溶性的腔肠素最高注射浓度可达500 µg/100 µl，在机体内能够达到一个相当高腔肠素水平，带来强烈的海肾荧光素信号。

【使用案例】

Yao M, Lu X, Lei Y, et al. Conditional Inducible Triple-Transgenic Mouse Model for Rapid Real-Time Detection of HCV NS3/4A Protease Activity.[J]. Plos One, 2016, 11(3).

3. Coelenterazin h 腔肠素h

【产品货号】cat#301 、CAS#50909-86-9

【产品规格】500µg、1mg、5mg、10mg

【产品简介】腔肠素h，是一种天然腔肠素的脱氧衍生物。当使用原水母发光蛋白时，腔肠素h的原始光输出量高于腔肠素的两倍；当使用水母萤光素酶时，腔肠素h的原始光输出量减少却有更长时间的持续性活跃。

【使用案例】

Charles R, Namkung Y, Cotton M, et al. βarrestin-Mediated Angiotensin II Signaling Controls the Activation of ARF6 and Endocytosis in Migration of Vascular Smooth Muscle Cells.[J]. Journal of Biological Chemistry, 2015.

Lahaie N, Kralikova M, Pr E L, et al. Post-endocytotic deubiquitination and degradation of the metabotropic γ-aminobutyric acid receptor by the ubiquitin specific protease 14.[J]. Journal of Biological Chemistry, 2016.

Beata J, Yuanyuan C, Tivadar O, et al. Disruption of Rhodopsin Dimerization with Synthetic Peptides Targeting an Interaction Interface.[J]. Journal of Biological Chemistry, 2015, 290(42):25728-44.

Gimenez L E, Baameur F, Vayttaden S J, et al. Salmeterol Efficacy and Bias in the Activation and Kinase-Mediated Desensitization of β2-Adrenergic Receptors[J]. Molecular pharmacology, 2015, 87(6): 954-964.

Hu J, Stern M, Gimenez L E, et al. A G Protein-Biased Designer G Protein-Coupled Receptor Useful for Studying the Physiological Relevance of Gq/11-Dependent Signaling Pathways.[J]. Journal of Biological Chemistry, 2016.

Giner X C, Cotnoir-White D, Mader S, et al. Selective ligand activity at Nur/retinoid X receptor complexes revealed by dimer-specific bioluminescence resonance energy transfer-based sensors.[J]. Faseb Journal, 2015, 29(10).

Lim J H, Park G C, Lee S M, et al. Surface-Tunable Bioluminescence Resonance Energy Transfer via Geometry-Controlled ZnO Nanorod Coordination[J]. Small, 2015, 11(28):3469-3475.

Robertson D N, Sleno R, Nagi K, et al. Design and construction of conformational biosensors to monitor ion channel activation: A prototype FlAsH/BRET-approach to Kir3 channels[J]. Environmental Modelling & Software, 2015, 8(9):A21.

Nakajima K I, Gimenez L E D, Gurevich V V, et al. Design and Analysis of an Arrestin-Biased DREADD[J]. Neuromethods, 2015, 108:29-48.

Lee J, Ghil S. Regulator of G protein signaling 8 inhibits protease-activated receptor 1/Gi/o signaling by forming a distinct G protein-dependent complex in live cells.[J]. Cellular Signalling, 2016, 28(5):391-400.

Zhao P, Lieu T, Barlow N, et al. Neutrophil elastase activates PAR2 and TRPV4 to cause inflammation and pain.[J]. Journal of Biological Chemistry, 2015.

Guixà-González R, Javanainen M, Gómez-Soler M, et al. Membrane omega-3 fatty acids modulate the oligomerisation kinetics of adenosine A2Aand dopamine D2receptors[J]. Scientific Reports, 2015, 6.

Chen Y, Hong T. High-throughput screening assays to identify small molecules preventing photoreceptor degeneration caused by the rhodopsin P23H mutation.[M]// Rhodopsin. Springer New York, 2015:369-390.

4. Coelenterazine native 天然腔肠素

【产品货号】cat#303、CAS#55779-48-1

【产品规格】500µg、1mg、5mg、10mg

【产品简介】腔肠素游离碱，纯度>95%。它是自然界最丰富的腔肠素，是海肾萤光素酶的首选底物。

【使用案例】

Franchi F, Peterson K M, Paulmurugan R, et al. Noninvasive Monitoring of the Mitochondrial Function in Mesenchymal Stromal Cells[J]. Molecular Imaging & Biology, 2016:1-9.

Yukiko H, Shuko T, Hansang C, et al. A Food and Drug Administration-approved asthma therapeutic agent impacts amyloid β in the brain in a transgenic model of Alzheimer disease.[J]. Journal of Biological Chemistry, 2015, 290(4):1966-1978.

Markova S V, Larionova M D, Burakova L P, et al. The smallest natural high-active luciferase: Cloning and characterization of novel 16.5-kDa luciferase from copepod Metridia longa[J]. Biochemical & Biophysical Research Communications, 2015, 457(1):77-82.

Abu‐Hayyeh S, Ovadia C, Lieu T M, et al. Prognostic and mechanistic potential of progesterone sulfates in intrahepatic cholestasis of pregnancy and pruritus gravidarum[J]. Hepatology, 2015.

Daniel W. Stuckey †, Shawn D. Hingtgen †, Karakas N, et al. Engineering Toxin-Resistant Therapeutic Stem Cells to Treat Brain Tumors[J]. Stem Cells, 2015, 33(2):589-600.

Duo X, Yang Z, Yanfeng Q, et al. Androgen Receptor Splice Variants Dimerize to Transactivate Target Genes.[J]. Cancer Research, 2015, 75(17):3663-71.

Zhang X, Ao Z, Bello A, et al. Characterization of the inhibitory effect of an extract of Prunella vulgaris on Ebola virus glycoprotein (GP)-mediated virus entry and infection.[J]. Antiviral Research, 2016, 127:20-31.

Piret J, Goyette N, Eckenroth B E, et al. Contrasting effects of W781V and W780V mutations in helix N of herpes simplex virus 1 and human cytomegalovirus DNA polymerases on antiviral drug susceptibility.[J]. Journal of Virology, 2015, 89(8):4636-4644.

A R Hacobian, K Posa-Markaryan, S Sperger, et al. Improved osteogenic vector for non-viral gene therapy[J]. European cells & materials, 2016, 31:191-204.

Savolainen M H, Xu Y, My?H?Nen T T, et al. Prolyl Oligopeptidase Enhances α-Synuclein Dimerization via Direct Protein-Protein Interaction.[J]. Journal of Biological Chemistry, 2015, 290(8):5117-26.

Leroy E, Defour J P, Sato T, et al. His499 Regulates Dimerization and Prevents Oncogenic Activation by Asparagine Mutations of the Human Thrombopoietin Receptor.[J]. Journal of Biological Chemistry, 2015, 20(2):268-279.

5. Coelenterazine 400a 腔肠素400a

【产品货号】cat#340、CAS#70217-82-2

【产品规格】500µg、1mg、10mg

【产品简介】可以被水母萤光素酶（Rluc)氧化，但不能被海肾萤光素酶氧化。它适用于实时检测钙离子、基因报告子实验以及BRET的首选底物。这种衍生物的最大激发波长约400nm，因此对对绿色荧光蛋白接收器的干扰最小。

【使用案例】

Namkung Y, Radresa O, Armando S, et al. Quantifying biased signaling in GPCRs using BRET-based biosensors[J]. Methods, 2015, 92:5-10.

6. GLuc GLOW Assay kit 海肾萤光素酶活性分析试剂盒

【产品货号】cat#320

【产品规格】50ml (约1000个反应）

【产品简介】它是一种新型的一步法GLOW分析试剂盒，适用于高通量筛选（HTS）。这种试剂盒包含一种能够延长Gaussia信号超过几个小时的缓冲液，Gaussia的半衰期在2-3小时之间。它的优势包括：一步法分析试剂（单一溶液包含裂解、稀释和反应缓冲液）、稳定的发光信号（2-3小时的半衰期）、检测分泌型Gaussia萤光素酶、检测胞内表达的Gaussia萤光素酶，以及低背景（高信号比）。

【使用案例】

Si C, Zhang Z, Wu Y, et al. Endoplasmic Reticulum Stress and Store-Operated Calcium Entry Contribute to Usnic Acid-Induced Toxicity in Hepatic Cells.[J]. Toxicological Sciences An Official Journal of the Society of Toxicology, 2015, 146(1).

7. Firefly Luciferin free acid 萤火虫荧光素游离酸

【产品货号】cat#306、CAS#2591-17-5

【产品规格】250mg、500mg、1g

【产品简介】纯度99%，这是一种游离酸，而不是钾盐或者钠盐等水溶性盐，需要使用碱性缓冲液溶解。

【使用案例】

Camille Saini †, Volodymyr Petrenko †, Pamela Pulimeno †, et al. A functional circadian clock is required for proper insulin secretion by human pancreatic islet cells[J]. Diabetes Obesity & Metabolism, 2015.

Tien-Jen L, Wen-Miin L, Pei-Wen H, et al. Rapamycin Promotes Mouse 4T1 Tumor Metastasis that Can Be Reversed by a Dendritic Cell-Based Vaccine.[J]. Plos One, 2015, 10(10).

Chang W T, Lai T H, Chyan Y J, et al. Specific Medicinal Plant Polysaccharides Effectively Enhance the Potency of a DC-Based Vaccine against Mouse Mammary Tumor Metastasis.[J]. Plos One, 2015, 10(3).

Lin T J, Lin H T, Chang W T, et al. Shikonin-enhanced cell immunogenicity of tumor vaccine is mediated by the differential effects of DAMP components[J]. Molecular Cancer, 2015, 14(1):1-13.

8. Coenzyme A 辅酶A

【产品货号】cat#309、CAS#85-61-0、PubMed#6816

【产品规格】250mg、500mg、1g

【产品简介】腺苷-3-磷酸-5-二磷酸-泛酸酯（还原型），能够延长萤火虫萤光素酶生物发光信号。

【使用案例】

Paul Friedman K, Watt ED, Hornung MW, et al. Tiered High-Throughput Screening Approach to Identify Thyroperoxidase Inhibitors within the ToxCast Phase I and II Chemical Libraries.[J]. Toxicological Sciences, 2016.

9. Firefly Luc Assay Reagent 萤火虫萤光素酶分析试剂

【产品货号】cat#318

【产品规格】100ml

【产品简介】一步法萤光素酶分析试剂盒，适用于检测萤火虫萤光素酶检测，尤其是高通量筛选中的萤光素酶活性检测。1000个反应。具有最明亮的信号强度，最好的信号稳定性。

【使用案例】

Sundeep M, Dolan T M, Maben Z J, et al. Adrenocorticotropic Hormone (ACTH) Responses Require Actions of the Melanocortin-2 Receptor Accessory Protein on the Extracellular Surface of the Plasma Membrane.[J]. Journal of Biological Chemistry, 2015, 290(46):27972-85.

Malik S, Dolan T M, Maben Z J, et al. ACTH Responses Require Actions of the Melanocortin-2 Receptor Accessory Protein on the Extracellular Surface of the Plasma Membrane.[J]. Journal of Biological Chemistry, 2015, 290(Suppl3):103-103(1).

10. Polyclonal Antisera-Gluc 萤光素酶多克隆抗血清

【产品货号】cat#401P

【产品规格】250 µl

【产品简介】Gaussia萤光素酶的多克隆兔抗体，效价>1:10,000。

【使用案例】

Farideh M G, Church D R, Schreiber C L, et al. Inhibitor of p52 NF-κB subunit and androgen receptor (AR) interaction reduces growth of human prostate cancer cells by abrogating nuclear translocation of p52 and phosphorylated ARser81[J]. Genes & Cancer, 2015, 6(9-10):428-444.

11. Coelenterazine F 腔肠素F

【产品货号】cat#345、CAS#123437-16-1

【产品规格】500µg、1mg、10mg

【产品简介】腔肠素F是原水母发光蛋白的首选底物。

【使用案例】

Malikova N P, Borgdorff A J, Vysotski E S. Semisynthetic photoprotein reporters for tracking fast Ca(2+) transients.[J]. Photochemical & Photobiological Sciences, 2015, 14(12).

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